FilterMax F5 Multi-Mode Microplate Reader. . 3. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. FITC-conjugated casein assay substrate was added to the wells and incubated at room temperature for 2 hours. Microplate Readers. 0. Perkin Elmer Wallac Victor2 1420 Multilabel. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. The optical performance is comparable to a top-of-the-line dedicated Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. c. HERM LB 500 with NaI detector Radio HPLC Detector. Neurotransmitter Transporter Uptake Assay Kit. 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). FilterMax F3 and F5 Multi-Mode Microplate Readers; Absorbance Readers. The Beta Plus version of the Sense uniquely combines liquid scintillation counting (LSC) with all common non-radiometric. IMAP assays are based on binding of. 00 USD. 7. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). 2. SpectraMax QuickDrop UV-Vis Spectrophotometer. please click here. SpectraMax Paradigm Multi-Mode Microplate Reader. SoftMax Pro GxP Compliance Software. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6% from 2020 to 2027. Readers ®Examples: Monochromators such as the SpectraMax i3 Multi-Mode Microplate Reader Detection Platform or filter based systems like the FilterMax™ F3 and F5 Multi-Mode Microplate Readers Luminescence Principle The total amount of light emitted is collected Plate specs White plates to reflect all the light to the detectorSpectraMax i3/i3x Multi-Mode Microplate Reader HeLa cells Cell viability assay SoftMax Pro Software Simplified homogenous experimental protocol The EarlyTox cell viability assay kits apply a no-wash, homogeneous protocol that simplifies the workflow and reduces the data variability typically encountered with multiple experimental steps. the luminescence reading was taken on a FilterMax F5 Multi-Mode. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. Images were captured using Molecular Devices CloneSelect Imager at a suitable time point post plating. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. 9 mM H 2 O 2 for. Plate Formats:. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. SoftMax® Pro Microplate Data Analysis Software provides integrated instrument control and data analysis. In the list below the slide number 21, select the positions where the emission filters are installedF3/F5 Multi-Mode Microplate Readers. Transfluor Technology. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Instrument Configuration: Assay Technology Adapta® & FilterMax F5 Multi-Mode Microplate Reader. Z’-LYTE® Compatible Microplate Reader Documentation Version No. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. 3 Pages. The hemolysis was calculated with the following formula:Luminescence from the converted ATP was then measured using a FilterMax F5 Multi-mode microplate reader (Molecular Devices). Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Dave Zeckhausen. The amount of colorimetric product was continuously monitored for 120 min. Citations: Dual monochromators increase performance and assay flexibility. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. 4 Pages. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. com A. 00 USD. Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. FAST AND ACCURATE METERTECH Microplate Reader $3,650. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. SP Bel-Art Petri Dish Plate Reader, Bel-Art Products, a part of SP . Luminescence (glow) Fluorescence polarization. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Different parameters (for instance, the incubation time of bacteriophage-MBs and S. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. The measured temperature can be used to provide feedback. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF. Categories:. Place plate in the GloMax® Instrument and read. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Microplate readers. In addition, the FilterMax F5 Reader is capable of measuring. FilterMax F3 & F5 Microplate Readers. Multi-mode Microplate Readers. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. 561. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. In addition, the instrument features linear and orbital shaking, plus temperature. Generally when this occurs, the destination BIG-IP device is unable to execute the tmsh command successfully. A microplate reader is laboratory machine used for the estimation of biochemical or. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Compact designs make these instruments. The results are expressed as cell viabilities (%) based on the control condition. shopping cart 0 items $0. 5% (v/v) to avoid toxicity. 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The amount of each HTRA1 protein was. FilterMax F5 Multi-Mode Microplate Reader. Share. 3 Pages. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. 4. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. FilterMax F5 Multi-Mode Microplate Reader. An array of these tubes is situated behind the sodium iodide crystal and may. Sunnyvale, Calif. SpectraMax iD5. 5 μg/ml) in the dark for 1 h. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. Molecular Devices’ FilterMaxTM F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. 4 Pages. 003 fmol 0. Synergy HTX laboratory equipment pdf manual download. Laser-aided direct metal deposition of metals and alloys. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). Equal concentrations of protein were separated in Mini-PROTEAN® TGX™ Precast 4–20% Gels (Bio-Rad, Hercules, CA, USA) and transferred to nitrocellulose. Multi-Mode Readers. 2. 5. S. This document is provided to customers. FilterMax F5 Multi-mode microplate reader. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. time plots at 30, 60, and 90 min using MATLAB ® R2017b (9. Watson. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. microplate reader. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. A Vision for the Future With TTP LabTech. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. --> Life sciences. FilterMax F5 Multi-Mode Microplate Reader. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. 8 Pages. Optical System: High energy xenon flash lamp. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Click to Contact Seller. , Sunnyvale, CA, USA). These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. They provide absorbance, fluorescence, glow luminescence, and other read modes for. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). 0. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. 00. 50/50. 003 fmol 0. 2. ** Some compatible filter sets are available for purchase from Life. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. All experiments were performed in triplicate. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Approximately 50 µl of these. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. 40266 Email: drugdiscoverytech@lifetech. The solution was incubated for 15 min and fluorescence measured on a SpectraMax microplate reader (Molecular Devices) with an. ** Some compatible filter sets are available for purchase from Life. FilterMax F3 & F5 Microplate Readers. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. The following document is intended to demonstrate setup of this instrument. Match case Limit results 1 per page. SpectraMax iD3 and iD5 Multi-Mode Microplate Readers;. FilterMax F5 Multi-Mode Microplate Reader. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Detection modalities are shown in Table 1-1. Time-resolved Fluorescence. 05); b Evaluation of the ATP levels of T. Not for use in diagnostic procedures. Buffer without protease was used as a blank for background subtraction. If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. Neurotransmitter Transporter Uptake Assay Kit. Recommended Optics P/N Filter information 6590-0048 FilterMax™ Fluorescence Excitation Filter 405 nm (bandwidth 30 nm) FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate Reader Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The measurement can be performed using a variety of detection technologies: the. They are very complex instruments. . Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 1. 3 Pages. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. The colorimetric signal development was stopped after 10 minutes. 2. 4 Pages. 3 Pages. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. Discover Precision and Reliability with Our Featured General Laboratory Instruments - Thermo Forma 3110 Water Jacketed CO2 Incubator, Thermo ULT185-5-V -80 Freezer, Filtermax F5 Multimode Microplate Reader, Spectramax M5e, Thermo Napco CO2 Incubator, and XS 64 Balance. --> Life sciences. ; Page 2 For research use only. Safety Information. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. 11 mg/mL in PBS, 20 h incubation) using the FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices (Sunnyvale, CA, USA), at 570 nm . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. Accurate measurements are ensured by automatic lamp calibration prior to each reading. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 4. SpectraMax® iD5 Multi-Mode Microplate Reader (Molecular Devices cat. The papers, which were published between 2010 and. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched value and flexibility for a variety of applications capable of performing: - Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. MetaFluor Software. By category. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. Visible absorbance -. Ultrafast Multi-Elemental Analysis Atomic Spectroscopy Consumables & Accessories. Cell viability was measured in percentages (%) as compared to untreated cells, which were assumed to be 100% viable. Assay buffer and 100% DMSO were used as vehicle controls. Plaque reduction. ScanLater Western Blot Detection System. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 666 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. FilterMax Multi-Mode Microplate Readers Functionality FilterMax F3 FilterMax F5 Absorbance (ABS ) Visible 4 4 Absorbance (ABS) UV 4 Fluorescence intensity (FI) top read 4 4 Fluorescence intensity (FI) bottom read 4 Luminescence (LUM) glow 4 4 Fluorescence polarization (FP) 4 Time-Resolved fluorescence (TRF) 4 Shaking 4 4 Temperature. 4 Pages. 6. ” e. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). , Ltd. An extra plate was done with all the serial dispersions for the MgO-NPs (in. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. , Sunnyvale, CA, USA) and normalized to the. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 3 Pages. 3 Pages. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. ABS Test results. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Molecular Devices FilterMax F5 User Manual (49 pages) Multi-Mode Microplate Readers. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 7°C (gradient heating design). Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. ScanLater Western Blot Detection System. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Turn on the power to the FilterMax F5. The USB adaptor comes with 1 X CD that contains its USB driver. All experiments were performed in triplicate. 3. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 3 Pages. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. Create an experiment based on the protocol. Users can program endpoint, kinetic, multiple wavelength, linear scan, and area scan measurement types. Add to cart. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. To read the absorbance, a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA 95134, USA) with a wavelength of 595 nm was used. 030 fmol Fluorescence Polarization (FP). The ucs load command creates a backup of. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. Approximately 50 μl of preculture were inoculated into 1 mL MMAB, leading to a starting density of 0. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Capabilities: FilterMax F5 Multi-mode microplate reader. Description: The FilterMax™ F3 features absorbance (visible),. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. 2 μM ATP standard was. LVF Monochromator™ : Excitation and Emission. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Renilla/Firefly luciferase activities were measured twice. A 1:1 binding ratio Hill model was used to fit the binding interaction data. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . MD, MPH, Milton J. Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. To do so, perform one of the. Click “Eject Plate Carrier. Page 154: Ordering Parts And Accessories. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). 00 USD. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 4 Pages. Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. Upgradeable modules are also available including western blot, cell imaging, and fast. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). Firefly and Renilla luciferase relative light units were measured using the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). By category. Renilla/Firefly luciferase activities were measured twice. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Transfluor Technology. At least three independent experiments were performed for each. I. SpectraMax Mini Multi-Mode Microplate Reader. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. MS-3 Laser Bar Code Reader. FilterMax F3 & F5 Multi-Mode Microplate Readers from. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. 8 Pages. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 3 software (Molecular Devices). 9. Search LabX. F5 has made an exception to this policy for BIG-IP 13. The values of luminescence are directly proportional to the number of viable cells. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). 561. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. $3,000 USD. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Hit enter to search or ESC to close. 100 fmol 1536-well (8 µL) 0. FilterMax F5 Multi-Mode Microplate Reader. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. Caspase-3 Activity. com A. Cover plate with gas-permeable membrane and put in incubator. com 8. VANTAstar™ Flexible Multi-mode Microplate Reader. The values of luminescence are directly proportional to the number of viable cells. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. 3 Pages. The ELISA test was performed using the supernatants from the morphological test. Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence.